Formulation and Evaluation of a Polyherbal Refreshing Drink with Antioxidant Potential

Abstract

The consumption of carbonated soft drinks containing high levels of sugar, artificial additives, and preservatives has been associated with various health problems including obesity, metabolic syndrome, and dental disorders. Herbal refreshing drinks represent a promising alternative as functional beverages that provide hydration along with therapeutic benefits derived from medicinal plants. The present study aimed to formulate and evaluate a polyherbal refreshing drink using selected medicinal herbs such as Mentha piperita, Ocimum sanctum, Zingiber officinale, Emblica officinalis, Citrus limon, Vaccinium myrtillus, and Citrus reticulata. Three formulations (F1, F2, and F3) were prepared by varying the concentrations of herbal extracts and sweetening agents. The formulations were evaluated for organoleptic characteristics, physicochemical parameters, phytochemical constituents, antioxidant activity using DPPH assay, and microbiological safety. The results revealed that all formulations exhibited acceptable sensory and physicochemical properties with pH ranging from 4.2 to 4.8 and total soluble solids between 8–12 °Brix. Phytochemical screening confirmed the presence of flavonoids, tannins, phenolic compounds, terpenoids, and alkaloids. Antioxidant evaluation demonstrated significant radical scavenging activity, with F3 showing the highest inhibition (82%), followed by F2 (74%). Microbiological analysis confirmed that all formulations were within permissible microbial limits. Among the tested formulations, F2 demonstrated the best balance between antioxidant potential and sensory acceptability. The developed polyherbal refreshing drink can serve as a natural functional beverage and a healthier alternative to synthetic soft drinks.