HPLC Method Development and Validation for the Simultaneous Estimation of Benserazide and Levodopa

Abstract

This research work outlines the advancement & verification of a HPLC technique for the parallel quantification of Benserazide& Levodopa. The chromatographic conditions were optimized, & SST parameters were evaluated, confirming compliance with established acceptance limits. The assay results showed a % assay of 99.55% for Benserazide& 101.32% for Levodopa. The technique exhibited linearity over the conc. extent of 10–50 µg per ml for both compounds, with R² of 0.9998 respectively. Precision & ID precision studies indicated low %RSD values, confirming the method’s reliability. Robustness testing revealed that variations in flow speed & MP composition. Limits of detection & quantification were determined, with LOD values of 0.05 ppm for Benserazide& 0.07 ppm for Levodopa. Overall, the verified HPLC technique is suitable for regular QA of Benserazide and Levodopa in formulations.