Effect of Physostigmine (an Organo Phosphate) on Biochemical variations of parotoid gland secretion and its extract of Bufo melanostictus (Schneider)

Abstract

The present study was carried out to investigate the biochemical variations of parotoid gland secretion and its extract in common Indian Toad Bufo melanostictus. The parotoid glands were injected with an organophosphate compound, the physostigmine and the quantitative variations were observed in proteins, carbohydrates and ninhydrine positive substances at different time intervals i.e., 4,8 and 12hrs. The results revealed that the components of proteins, carbohydrates and ninhydrine positive substances were found to be decreased significantly at 4,8 and 12hrs time interval of Physostigmine in parotoid gland secretion compared to gland extract. The gradual decrease observed in biochemical constituents of both parotoid gland secretion and gland extract reveals triplicate response denoting the detoxification nature of OP compound i.e., Physostigmine. The maximum decrease in proteins followed by ninhydrin positive substances (free amino acids) and carbohydrates was observed at 4hrs and 12hrs compared to 8hrs time interval and control in both parotoid gland secretion and its extract.