Journal of Pharmaceutical and Biomedical Analysis Letters
type: channel
publisher: Pharma Research Library
language: en
publication name: Journal of Pharmaceutical and Biomedical Analysis Letters
issn: 2347-4742
items: An Observational Study on Clinical Profile & Etiological Factors and Complications of Liver Function Tests
items: Simultaneous Estimation of Drugs in their Combined Dosage forms by UV-Visible Spectroscopy
items: Development and Evaluation of Topical herbal Formulation for anti Inflammatory Activity
items: Formulation and Evaluation of Ivabradine Oral Thin Films
items: Biosynthesis of silver nano particles of herbal plant extracts and characterisation by using UV and FTIR analysis
items: AI-Assisted Identification and Quantification of Pharmaceutical Impurities using HPLC Chromatographic Data
items: Phytoconstituents as Potential Anticancer Agents: Mechanisms, Evidence, and Therapeutic Prospects
items: Development and Validation of a Liquid Chromatography Method for the Simultaneous Estimation of Sulfadoxine and Pyrimethamine in Combined Dosage Forms
items: QBD-Based Analytical Method Development and Validation for the Simultaneous Estimation of Voglibose and Metformin Using RP-HPLC
items: QBD-based RP-HPLC Method Development and Validation for Thioacetazone and Isoniazide in Pharmaceutical Dosage Forms as per ICH Guidelines
items: New Validated Stability Indicating RP-HPLC Method Development for Simultaneously Estimation of Betamethasone and Calcipotriene in Its Bulk and Pharmaceutical Dosage Form
items: Method Development and Validation for the Simultaneous Estimation of Sequinavir and Tipronavir by using HPLC
items: RS Method for Quantification of Imeglimin HCl in Its Pure and Tablet Dosage form by using RP-HPLC Technology
items: Preparation and Evaluation of Floating Beads of Propranolol Hydrochloride
items: Novel Therapeutic Options for Prevention and Management of Cancer
items: Investigation of Antidiabetic and Anti-inflammatory Properties of Ghee through GC–MS Analysis
items: Analytical Method Development and Validation for Simultaneous Estimaton of Azelnidipine and Telmisartan by RP-HPLC in Bulk and Tablet Dosage Forms
items: Integrated Analytical Quality by Design Approach for the Development and Validation of Liquid Chromatography Method for Simulataneous Estimation of Sulfadoxane and Pyrimethamine
items: RP-HPLC Method Development and Validation for the simultaneous estimation of Meropenem and Vaborbactam in its pure and Dosage form
items: RP-HPLC Method Development and Validation of a Stability Indicating Cidofovir in Its Pure and Dosage Form
items: Screening the efficacy of compounds from Ghee to control cancer through GC-MS Analysis
items: Development of a Robust RP-HPLC Method for the Analysis of Ofloxacin and Nitazoxanide in Fixed-Dose Combination Products
items: Development and Validation of a New RP-HPLC Method for the Estimation of Citalopram in Tablet Dosage Forms

An Observational Study on Clinical Profile & Etiological Factors and Complications of Liver Function Tests
type: item
description: The present study finds out the etiological causes and clinical profile data for liver function tests. Monitoring and managing of liver enzyme levels in different liver disease find out the etiological causes of different liver diseases. Measure the severity of a disease, particularly scarring of the liver (cirrhosis). To Monitor the progression of a disease, such as viral or alcoholic hepatitis, and determine how well a treatment is working. The study concluded that we have collected the data from the hospitalized patients. We have analyzed the data according to the study and determined above. Our study is about estimation of liver functional tests and causes of elevated liver functional changes by different parameters. The clinical pharmacist and family members has to educate the people regarding the complications and consequences associated with liver function tests and serious causes with elevated levels. Awareness has to be created to the public due to elevation of levels to make the society and health mission will fulfill by promote awareness among society.
subject: Articles
  topic: section
subject: Observational Study
  topic: keywords
subject: Liver
  topic: keywords
subject: Silver nanoparticles
  topic: keywords
subject: Clinical Profile
  topic: keywords
subject: Etiological Factors
  topic: keywords
subject: Complications
  topic: keywords
creator: SK. Meharunnisa
creator: N. Bala Jahnavi
creator: Afroz Patan
creator: Yadala Prapurna Chandra
rights: Copyright (c) 2026 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2026-07-14
publication date: 2026-07-14
starting page: 42
ending page: 46
doi: 10.30904/j.jpbmal.2026.5025

Simultaneous Estimation of Drugs in their Combined Dosage forms by UV-Visible Spectroscopy
type: item
description: The present study aims to develop and validate simple, accurate, precise, rapid, and economical UV–Visible spectrophotometric methods for the simultaneous estimation of selected multicomponent pharmaceutical formulations. With the increasing use of combination drug therapy for enhanced therapeutic efficacy and patient compliance, the need for reliable analytical methods has become essential. In this work, simultaneous equation and absorbance ratio methods were developed for the estimation of drug combinations such as Ofloxacin–Tinidazole, Alprazolam–Propranolol, and Amlodipine–Metoprolol. The developed methods are based on the measurement of absorbance at selected wavelengths, including λmax and isoabsorptive points, and obey Beer–Lambert’s law within the specified concentration ranges. Various analytical parameters such as linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ) were evaluated in accordance with standard validation guidelines. The results demonstrated good correlation coefficients, high recovery values close to 100%, and low %RSD values, indicating excellent reproducibility and reliability. The proposed methods were successfully applied for the analysis of pharmaceutical formulations without interference from excipients. Due to their simplicity, sensitivity, and cost-effectiveness, these methods can be effectively used for routine quality control analysis of multicomponent drug formulations in pharmaceutical industries and laboratories.
subject: Articles
  topic: section
subject: UV–VIS spectrophotometry
  topic: keywords
subject: Beer–Lambert’s law
  topic: keywords
subject: Pharmaceutical analysis
  topic: keywords
subject: Method validation
  topic: keywords
subject: Drug combinations
  topic: keywords
subject: Quality control
  topic: keywords
creator: CH. A.L. Kalyani
creator: E. Bhavani
creator: G. Koushik
creator: K. Anitha
creator: Y. Sireesha
rights: Copyright (c) 2026 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2026-03-15
publication date: 2026-03-15
starting page: 21
ending page: 24
doi: 10.30904/j.jpbmal.2026.4953

Development and Evaluation of Topical herbal Formulation for anti Inflammatory Activity
type: item
description: The present study was undertaken to develop and evaluate novel and conventional topical herbal formulations for anti-inflammatory activity using selected medicinal plants, namely Curcuma longa, Epilobium angustifolium, and Agathosma betulina. These plants were selected based on ethnomedicinal relevance and were authenticated prior to use. The extracts were subjected to pharmacognostic, phytochemical, and physicochemical standardization, confirming the presence of important bioactive constituents such as phenolics, tannins, terpenoids, and flavonoids, and ensuring compliance with WHO guidelines for safety and quality. Topical formulations including conventional creams, transdermal patches, and topical film-forming solutions were developed using a 3² full factorial design to optimize formulation variables. The prepared formulations were evaluated for physicochemical parameters such as pH, viscosity, homogeneity, spreadability, mechanical strength, moisture content, and film integrity. In vitro drug release studies were performed using suitable diffusion techniques, and release kinetics were analyzed using various mathematical models. The optimized formulations exhibited desirable physicochemical properties and demonstrated sustained and controlled drug release profiles. Kinetic modeling indicated that drug release followed diffusion-controlled mechanisms, predominantly Higuchi and Korsmeyer–Peppas models. The findings of the study suggest that the developed formulations are stable, effective, and suitable for topical delivery of herbal actives. In conclusion, the study highlights the potential of combining traditional herbal knowledge with modern formulation strategies to develop safe, efficient, and patient-compliant topical anti-inflammatory drug delivery systems.
subject: Articles
  topic: section
subject: Topical herbal formulations
  topic: keywords
subject: Anti-inflammatory activity
  topic: keywords
subject: Curcuma longa
  topic: keywords
subject: Epilobium angustifolium
  topic: keywords
subject: Agathosma betulina
  topic: keywords
creator: Ramya Nagasuri
creator: C. Uday Kumar
creator: M. Karthik
creator: M. Arsita
creator: P. Abinaya
creator: S. Arshad
rights: Copyright (c) 2026 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2026-03-10
publication date: 2026-03-10
starting page: 15
ending page: 20
doi: 10.30904/j.jpbmal.2026.4932

Formulation and Evaluation of Ivabradine Oral Thin Films
type: item
description: In present study oral thin films of Ivabradine were developed to have a faster on set of action. The oral thin films were developed by using polymers HPMC E5, HPMC E 15 and PVP K90.Oral thin films were prepared by employing solvent casting method. Propylene glycol was selected as permeation enhancer and plasticizer. Drug excipient compatibility studies were carried out by using FTIR, and it was observed that there were no interactions. Formulations were prepared with the varying concentrations polymers ranging from F1-F6, and all the formulations were evaluated for various physical parameters Physical appearance, Weight variation, Thickness, Folding endurance, Tensile strength, Drug content, Moisture uptake, Moisture content and all the results were found to be were found to be with in the pharmacopeial limits, in-vitro drug release studies by using dialysis membrane. Among all the 6 formulations F1 formulation which contain HPMC E15 300mg and shown 97.2.% cumulative drug release within 30 min. And compared to HPMC E15, HPMC E5 and PVP K90, HPMC E 15 showed better drug release profile.
subject: Articles
  topic: section
subject: Ivabradine
  topic: keywords
subject: HPMC E15
  topic: keywords
subject: HPMC E5
  topic: keywords
subject: PVP K90
  topic: keywords
creator: Manjula Katkuri
creator: Ramyakrishna Samala
creator: Aleti PR
rights: Copyright (c) 2026 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2026-06-20
publication date: 2026-06-20
starting page: 38
ending page: 41
doi: 10.30904/j.jpbmal.2026.5024

Biosynthesis of silver nano particles of herbal plant extracts and characterisation by using UV and FTIR analysis
type: item
description: The present study explores the green synthesis of silver nanoparticles (AgNPs) using aqueous extracts of Zingiber officinale (ginger rhizome), Carica papaya (papaya seeds), and Camellia sinensis (green tea leaves). Extracts were subjected to phytochemical screening, total phenolic content (TPC) estimation, and characterization of AgNPs by UV–Vis and FTIR. Their biological efficacy was evaluated through catalytic degradation of methylene blue, antioxidant activity (DPPH assay), and antimicrobial studies. Ginger showed the highest TPC (75.65 mg GAE/mg), efficient AgNP formation, and strong antioxidant, antimicrobial, and catalytic activities. Papaya seeds, with lower TPC (13.7 mg GAE/mg), produced AgNPs with moderate antioxidant and antimicrobial effects, particularly against E. coli. Green tea (36.64 mg GAE/mg) yielded stable AgNPs with significant antioxidant, catalytic, and antibacterial activity. These findings highlight plant-mediated AgNPs as eco-friendly nanomaterials with biomedical and environmental potential, with ginger demonstrating the strongest overall performance.
subject: Articles
  topic: section
subject: Green synthesis
  topic: keywords
subject: Silver nanoparticles
  topic: keywords
subject: Zingiber officinale
  topic: keywords
subject: Carica papaya
  topic: keywords
subject: Camellia sinensis
  topic: keywords
subject: Antioxidant
  topic: keywords
creator: Batchu Malathi
creator: Mucharla Pradeep Kumar
creator: Bollu Jyothsna
creator: Shaik Gousia
rights: Copyright (c) 2026 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2026-06-20
publication date: 2026-06-20
starting page: 33
ending page: 37
doi: 10.30904/j.jpbmal.2026.5023

AI-Assisted Identification and Quantification of Pharmaceutical Impurities using HPLC Chromatographic Data
type: item
description: Pharmaceutical impurities present in active pharmaceutical ingredients (APIs) and finished drug products can have profound impacts on drug safety, efficacy, and stability. Regulatory authorities such as the International Council for Harmonisation (ICH), U.S. Food and Drug Administration (FDA), and European Medicines Agency (EMA) require stringent impurity identification and quantification to ensure drug quality and patient safety. High-Performance Liquid Chromatography (HPLC) remains the most common analytical method for impurity profiling, thanks to its high sensitivity, precision, and ability to separate complex mixtures. However, traditional HPLC data interpretation relies heavily on manual analysis, making it time-consuming, operator-dependent, and prone to human error. Artificial Intelligence (AI) and Machine Learning (ML) have emerged as transformative tools for automating chromatographic data analysis, enabling rapid and accurate impurity detection, identification, and quantification. AI algorithms can efficiently process large chromatographic datasets, identify subtle peak patterns, correct baseline drifts, and predict retention behaviours under various chromatographic conditions. This comprehensive article explores the integration of AI approaches into HPLC impurity profiling, covering theoretical foundations, data preprocessing, peak recognition, automated quantification, and future implications for pharmaceutical quality control and regulatory compliance.
subject: Articles
  topic: section
subject: Artificial Intelligence (AI)
  topic: keywords
subject: High-Performance Liquid Chromatography (HPLC)
  topic: keywords
subject: Pharmaceutical Impurities
  topic: keywords
subject: Chemometrics
  topic: keywords
subject: Machine Learning (ML)
  topic: keywords
creator: Abdul Basith J
creator: Brindha Shree V
creator: Balagopal J
creator: Dharshini P
creator: Jai Surya I
creator: Ravisankar Rathesan
creator: Nataraj Palaniyappan
rights: Copyright (c) 2026 Authors https://creativecommons.org/licenses/by-nc/4.0
date: 2026-05-25
publication date: 2026-05-25
starting page: 28
ending page: 32
doi: 10.30904/j.jpbmal.2026.4991

Phytoconstituents as Potential Anticancer Agents: Mechanisms, Evidence, and Therapeutic Prospects
type: item
description: Cancer remains a major global health challenge despite advances in diagnosis and treatment. Conventional therapies such as chemotherapy and radiotherapy are often limited by systemic toxicity, resistance development, and damage to healthy tissues. Phytoconstituents, bioactive secondary metabolites derived from medicinal plants, have gained considerable attention as potential anticancer agents due to their multi-targeted mechanisms, relative safety, and chemopreventive properties. This study reviews the classification, molecular mechanisms, experimental evidence, clinical relevance, advantages, and limitations of phytoconstituents in cancer therapy. Evidence from in vitro, in vivo, and clinical studies indicates that compounds such as curcumin, resveratrol, epigallocatechin gallate (EGCG), genistein, sulforaphane, silymarin, vincristine, vinblastine, and paclitaxel modulate key signaling pathways including NF-κB, PI3K/Akt, MAPK, p53, VEGF, and STAT3. These compounds exert anticancer effects through apoptosis induction, cell cycle arrest, inhibition of angiogenesis, suppression of metastasis, epigenetic modulation, and targeting of cancer stem cells. Although limitations such as poor bioavailability and insufficient large-scale clinical validation persist, advancements in nanotechnology and targeted drug delivery systems offer promising solutions. Phytoconstituents represent a valuable bridge between traditional medicine and modern oncology and may contribute significantly to future cancer prevention and therapy strategies.
subject: Articles
  topic: section
subject: Phytoconstituents
  topic: keywords
subject: Cancer therapy
  topic: keywords
subject: Apoptosis
  topic: keywords
subject: Resveratrol
  topic: keywords
subject: Natural products
  topic: keywords
creator: Salma Sultana SK
creator: Swarupa Rani R
creator: Venkata Harsha Vardan D
creator: Likitha P
creator: Ashok Damai
rights: Copyright (c) 2026 Authors https://creativecommons.org/licenses/by-nc/4.0
date: 2026-05-25
publication date: 2026-05-25
starting page: 25
ending page: 27
doi: 10.30904/j.jpbmal.2026.4949

Development and Validation of a Liquid Chromatography Method for the Simultaneous Estimation of Sulfadoxine and Pyrimethamine in Combined Dosage Forms
type: item
description: The final method employed a Platisil C18-ODS column (250×4.6 mm, 5 µm) with a mobile phase of 50% 0.1% formic acid (pH 4.5) and 50% methanol, at a flow rate of 1.0 ml/min, and UV detection at 230 nm. System suitability parameters such as retention time, theoretical plates, and tailing factor were within acceptable limits, confirming the method’s efficiency and reliability. Validation was performed according to ICH Q2(R1) guidelines, demonstrating excellent analytical performance. Assay results showed drug content of 99.1% for Sulfadoxine and 98.6% for Pyrimethamine. Linearity was observed over wide concentration ranges with correlation coefficients of 0.9997 and 0.9996, respectively. Precision and intermediate precision studies yielded %RSD values below 2%, indicating high repeatability and ruggedness. Recovery studies confirmed accuracy within the 98–102% range, while LOD and LOQ values (0.02 µg/ml for Sulfadoxine and 0.05µg/ml for Pyrimethamine) highlighted the method’s sensitivity. Robustness testing under slight variations in flow rate and mobile phase composition showed consistent results, affirming the method’s suitability for routine quality control of combined dosage formulations.
subject: Articles
  topic: section
subject: RP-HPLC
  topic: keywords
subject: Pyrimethamine
  topic: keywords
subject: Sulfadoxine
  topic: keywords
subject: ICH
  topic: keywords
creator: Sai Kiran V
creator: Chaitanya Prasad K
creator: Nagasree K
creator: Shravan Kumar K
rights: Copyright (c) 2026 Authors https://creativecommons.org/licenses/by-nc/4.0
date: 2026-02-25
publication date: 2026-02-25
starting page: 11
ending page: 14
doi: 10.30904/j.jpbmal.2026.4926

QBD-Based Analytical Method Development and Validation for the Simultaneous Estimation of Voglibose and Metformin Using RP-HPLC
type: item
description: A scientifically rigorous and precise reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed using a Quality by Design (QbD) approach for the simultaneous estimation of Voglibose and Metformin in fixed-dose combination formulations. The method was systematically optimized through Design of Experiments (DoE), evaluating critical parameters such as flow rate, buffer pH, and organic solvent ratio for their impact on chromatographic responses like resolution and tailing factor. Statistical analysis via ANOVA confirmed the significance of the quadratic model in achieving optimal separation. The final method employed a Zorbax C18 column with phosphate buffer (pH 4.5) and acetonitrile (45:55 v/v) as the mobile phase, operating at a flow rate of 1.2 mL/min and detection at 225 nm. This setup yielded excellent peak separation with a resolution of 10.10 between the analytes. Validation was conducted in accordance with ICH Q2(R1) guidelines, demonstrating outstanding linearity for Metformin (25–125 µg/mL) and Voglibose (0.1–0.5µg/mL), with correlation coefficients of 0.9998 and 0.9994, respectively. Precision studies confirmed repeatability and intermediate precision (%RSD < 2%), while accuracy was validated through high recovery rates (99.4% for Voglibose and 99.6% for Metformin). Sensitivity was affirmed with acceptable LOD and LOQ values. This QbD-driven RP-HPLC method provides a robust, validated, and regulatory-compliant solution for simultaneous estimation of Voglibose and Metformin in pharmaceutical applications
subject: Articles
  topic: section
subject: RP-HPLC
  topic: keywords
subject: Voglibose
  topic: keywords
subject: Metformin
  topic: keywords
subject: ICH
  topic: keywords
subject: QBD
  topic: keywords
creator: Manjula B
creator: Chaitanya Prasad K
creator: Nagasree K
creator: Shravan Kumar K
rights: Copyright (c) 2026 Authors https://creativecommons.org/licenses/by-nc/4.0
date: 2026-02-25
publication date: 2026-02-25
starting page: 06
ending page: 10
doi: 10.30904/j.jpbmal.2026.4925

QBD-based RP-HPLC Method Development and Validation for Thioacetazone and Isoniazide in Pharmaceutical Dosage Forms as per ICH Guidelines
type: item
description: A novel and comprehensive High-Performance Liquid Chromatography (HPLC) method was developed and optimized for the simultaneous estimation of Isoniazid and Thioacetazone in pharmaceutical formulations. Method optimization was achieved using a Box-Behnken design coupled with response surface methodology, evaluating critical chromatographic parameters such as flow rate, buffer ratio, and buffer pH. The optimized conditions employed a Platisil C18 column (150×3.0 mm, 3μm) with a mobile phase of 0.1% orthophosphoric acid buffer (pH 3.5) and methanol (40:60 v/v), at a flow rate of 0.8 mL/min and detection at 258 nm. System suitability tests confirmed acceptable resolution (≥2), tailing factor (≤2), and theoretical plate count (≥2000), ensuring reliable separation and peak symmetry for both analytes. The method was rigorously validated in accordance with ICH guidelines, demonstrating excellent linearity for Isoniazid (10–50 µg/mL, R² = 0.9998) and Thioacetazone (5–25 µg/mL, R² = 0.9995). Precision studies yielded %RSD values below 2%, confirming reproducibility under varied conditions. Sensitivity was established through low LOD and LOQ values, while recovery studies at multiple concentration levels (50%, 100%, 150%) showed accuracy within 98–102%. Robustness was maintained despite deliberate variations in chromatographic parameters. Overall, the validated method is accurate, precise, sensitive, and robust, making it highly suitable for routine quality control of Isoniazid and Thioacetazone in pharmaceutical dosage forms.
subject: Articles
  topic: section
subject: RP-HPLC
  topic: keywords
subject: Isoniazid
  topic: keywords
subject: Thioacetazone
  topic: keywords
subject: Platisil C18 column
  topic: keywords
subject: orthophosphoric acid buffer
  topic: keywords
creator: Priyanka K
creator: Chaitanya Prasad K
creator: Nagasree K
creator: Shravan Kumar K
rights: Copyright (c) 2026 Authors https://creativecommons.org/licenses/by-nc/4.0
date: 2026-02-25
publication date: 2026-02-25
starting page: 01
ending page: 05
doi: 10.30904/j.jpbmal.2026.4921

New Validated Stability Indicating RP-HPLC Method Development for Simultaneously Estimation of Betamethasone and Calcipotriene in Its Bulk and Pharmaceutical Dosage Form
type: item
description: This study focused on developing and characterizing floating microspheres of Propranolol HCl, aiming to enhance gastric retention and sustained drug release. A UV-Visible spectrophotometric method was validated for drug quantification in 0.1N HCl at 256 nm, exhibiting strong linearity over the concentration range of 2 to 10 µg/ml. Compatibility studies using FTIR spectroscopy confirmed no chemical interactions between the drug and polymers, ensuring formulation stability. The prepared microspheres showed high percentage yields between 81.58% and 95.50%, with drug entrapment efficiency improving as polymer concentration increased due to higher solution viscosity reducing drug diffusion during formation. Further evaluations revealed that the microspheres exhibited satisfactory swelling behavior and mucoadhesive properties essential for prolonged gastric residence. In vitro release experiments demonstrated efficient and controlled drug liberation over 12 hours, with release kinetics fitting diffusion-controlled models such as zero-order, first-order, Higuchi, and Korsmeyer-Peppas. Optimized formulations showed promising potential for sustained release and enhanced gastroretention, which could improve therapeutic effectiveness and patient adherence.
subject: Articles
  topic: section
subject: Propranolol HCl
  topic: keywords
subject: floating microspheres
  topic: keywords
subject: UV-Visible spectrophotometry
  topic: keywords
subject: sustained release
  topic: keywords
subject: mucoadhesion
  topic: keywords
subject: drug release kinetics
  topic: keywords
subject: FTIR compatibility
  topic: keywords
creator: Naveen S
creator: Praveena T
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-10-05
publication date: 2025-10-05
starting page: 77
ending page: 85
doi: 10.30904/j.jpbmal.2025.4905

Method Development and Validation for the Simultaneous Estimation of Sequinavir and Tipronavir by using HPLC
type: item
description: This study focused on developing and characterizing floating microspheres of Propranolol HCl, aiming to enhance gastric retention and sustained drug release. A UV-Visible spectrophotometric method was validated for drug quantification in 0.1N HCl at 256 nm, exhibiting strong linearity over the concentration range of 2 to 10 µg/ml. Compatibility studies using FTIR spectroscopy confirmed no chemical interactions between the drug and polymers, ensuring formulation stability. The prepared microspheres showed high percentage yields between 81.58% and 95.50%, with drug entrapment efficiency improving as polymer concentration increased due to higher solution viscosity reducing drug diffusion during formation. Further evaluations revealed that the microspheres exhibited satisfactory swelling behavior and mucoadhesive properties essential for prolonged gastric residence. In vitro release experiments demonstrated efficient and controlled drug liberation over 12 hours, with release kinetics fitting diffusion-controlled models such as zero-order, first-order, Higuchi, and Korsmeyer-Peppas. Optimized formulations showed promising potential for sustained release and enhanced gastroretention, which could improve therapeutic effectiveness and patient adherence.
subject: Articles
  topic: section
subject: Propranolol HCl
  topic: keywords
subject: floating microspheres
  topic: keywords
subject: UV-Visible spectrophotometry
  topic: keywords
subject: sustained release
  topic: keywords
subject: mucoadhesion
  topic: keywords
subject: drug release kinetics
  topic: keywords
subject: FTIR compatibility
  topic: keywords
creator: Yash Raj Kiran Bhalerao
creator: Aravinda Reddy P
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-09-25
publication date: 2025-09-25
starting page: 69
ending page: 76
doi: 10.30904/j.jpbmal.2025.4899

RS Method for Quantification of Imeglimin HCl in Its Pure and Tablet Dosage form by using RP-HPLC Technology
type: item
description: This study focused on developing and characterizing floating microspheres of Propranolol HCl, aiming to enhance gastric retention and sustained drug release. A UV-Visible spectrophotometric method was validated for drug quantification in 0.1N HCl at 256 nm, exhibiting strong linearity over the concentration range of 2 to 10 µg/ml. Compatibility studies using FTIR spectroscopy confirmed no chemical interactions between the drug and polymers, ensuring formulation stability. The prepared microspheres showed high percentage yields between 81.58% and 95.50%, with drug entrapment efficiency improving as polymer concentration increased due to higher solution viscosity reducing drug diffusion during formation. Further evaluations revealed that the microspheres exhibited satisfactory swelling behavior and mucoadhesive properties essential for prolonged gastric residence. In vitro release experiments demonstrated efficient and controlled drug liberation over 12 hours, with release kinetics fitting diffusion-controlled models such as zero-order, first-order, Higuchi, and Korsmeyer-Peppas. Optimized formulations showed promising potential for sustained release and enhanced gastroretention, which could improve therapeutic effectiveness and patient adherence.
subject: Articles
  topic: section
subject: Propranolol HCl
  topic: keywords
subject: floating microspheres
  topic: keywords
subject: UV-Visible spectrophotometry
  topic: keywords
subject: sustained release
  topic: keywords
subject: mucoadhesion
  topic: keywords
subject: drug release kinetics
  topic: keywords
subject: FTIR compatibility
  topic: keywords
creator: Kailas Pagar Ojas
creator: Suhasini B
creator: Aravinda Reddy P
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-09-18
publication date: 2025-09-18
starting page: 62
ending page: 68
doi: 10.30904/j.jpbmal.2025.4898

Preparation and Evaluation of Floating Beads of Propranolol Hydrochloride
type: item
description: This study focused on developing and characterizing floating microspheres of Propranolol HCl, aiming to enhance gastric retention and sustained drug release. A UV-Visible spectrophotometric method was validated for drug quantification in 0.1N HCl at 256 nm, exhibiting strong linearity over the concentration range of 2 to 10 µg/ml. Compatibility studies using FTIR spectroscopy confirmed no chemical interactions between the drug and polymers, ensuring formulation stability. The prepared microspheres showed high percentage yields between 81.58% and 95.50%, with drug entrapment efficiency improving as polymer concentration increased due to higher solution viscosity reducing drug diffusion during formation. Further evaluations revealed that the microspheres exhibited satisfactory swelling behavior and mucoadhesive properties essential for prolonged gastric residence. In vitro release experiments demonstrated efficient and controlled drug liberation over 12 hours, with release kinetics fitting diffusion-controlled models such as zero-order, first-order, Higuchi, and Korsmeyer-Peppas. Optimized formulations showed promising potential for sustained release and enhanced gastroretention, which could improve therapeutic effectiveness and patient adherence.
subject: Articles
  topic: section
subject: Propranolol HCl
  topic: keywords
subject: floating microspheres
  topic: keywords
subject: UV-Visible spectrophotometry
  topic: keywords
subject: sustained release
  topic: keywords
subject: mucoadhesion
  topic: keywords
subject: drug release kinetics
  topic: keywords
subject: FTIR compatibility
  topic: keywords
creator: Niharika Chilukuri
creator: Rajkumar Devara
creator: Aravinda Reddy P
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-09-12
publication date: 2025-09-12
starting page: 54
ending page: 61
doi: 10.30904/j.jpbmal.2025.4885

Novel Therapeutic Options for Prevention and Management of Cancer
type: item
description: The high incidence of cancer may be caused by several factors, such as genetic mutations, environmental factors, insufficient physical activity, diverse lifestyles, unstable behaviors related to diet, smoking, and alcohol consumption. The current treatment methods for different stages of various cancers include chemotherapy, radiation therapy, and surgical procedures for solid tumors, or a combination of the above. Although these different treatment modalities can effectively reduce cancer, patients may also experience side effects. Radiation therapy runs the risk of causing DNA damage in surrounding healthy cells, which could potentially lead to new incidences of cancer. Similarly, although surgical intervention the primary treatment for solid tumors significantly improves patient survival, its success rate depends on the expertise of the surgeon and the availability of screening methods, including hospital imaging equipment. The introduction of chemotherapy was a milestone in cancer treatment. However, prolonged use of chemotherapy drugs, especially those affecting tumor cell metabolic pathways and signal transduction, can influence tumor occurrence, metastasis, drug response, recurrence, drug resistance, and cancer stem cells (CSCs). Traditionally, drug development involves preclinical research and clinical trials. Preclinical studies involve testing the efficacy, toxicity, pharmacokinetics, and pharmacodynamics of drugs in human tumor cells and animal models. Once the therapeutic efficacy of a drug has been determined, the drug moves into the clinical trial phase, which includes Phase I, II, and III human clinical trials, to determine the safety and effectiveness of the drug. As such, it takes 10–15 years and costs $1–2 billion to produce a new drug approved for clinical use.
subject: Articles
  topic: section
subject: Cancer
  topic: keywords
subject: chemotherapy
  topic: keywords
subject: tumor occurrence
  topic: keywords
subject: metastasis
  topic: keywords
subject: clinical trials
  topic: keywords
subject: DNA damage
  topic: keywords
creator: Gnana Prasuna G
creator: Venkata Lava Kumar Reddy Kuraku
creator: Sivamani Dola
creator: Gowri Shankar V
creator: Uday B.T
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-08-24
publication date: 2025-08-24
starting page: 46
ending page: 53
doi: 10.30904/j.jpbmal.2025.4851

Investigation of Antidiabetic and Anti-inflammatory Properties of Ghee through GC–MS Analysis
type: item
description: Ghee, a clarified form of butter traditionally used in Indian cooking and Ayurvedic medicine, has gained attention for its potential therapeutic benefits. This study explores the antidiabetic and anti-inflammatory properties of ghee through a comprehensive review of preclinical and clinical literature, alongside possible biochemical mechanisms. Ghee is rich in short-chain and medium-chain fatty acids, conjugated linoleic acid (CLA), and fat-soluble vitamins, which contribute to its bioactivity. Antidiabetic effects are primarily attributed to improved insulin sensitivity, modulation of lipid metabolism, and reduction in oxidative stress. Ghee has also demonstrated anti-inflammatory properties through the down regulation of pro-inflammatory cytokines and inhibition of COX-2 expression. These effects may support ghee’s role as a functional dietary component in managing chronic metabolic disorders.
subject: Articles
  topic: section
subject: Ghee
  topic: keywords
subject: Antidiabetic activity
  topic: keywords
subject: Anti-inflammatory properties
  topic: keywords
subject: Conjugated linoleic acid
  topic: keywords
subject: Insulin sensitivity
  topic: keywords
subject: Oxidative stress
  topic: keywords
subject: Ayurvedic medicine
  topic: keywords
subject: Functional food
  topic: keywords
subject: Chronic inflammation
  topic: keywords
subject: Lipid metabolism
  topic: keywords
creator: Siva Prasad K
creator: Mahesh G
creator: Harika D
creator: Mounika B
creator: Anuradha C
creator: Ganesh Kumar Reddy V
creator: Siva G
creator: Veeraraghavulu T
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-06-30
publication date: 2025-06-30
starting page: 37
ending page: 40
doi: 10.30904/j.jpbmal.2025.4826

Analytical Method Development and Validation for Simultaneous Estimaton of Azelnidipine and Telmisartan by RP-HPLC in Bulk and Tablet Dosage Forms
type: item
description: A new, simple, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validation of Azelnidipine and Telmisartan in its pure form as well as in combined marketed formulation. Chromatography was carried out on a Phenomenex Luna C18 (4.6mm×250mm) 5µm particle size column using a mixture of Methanol: Phosphate Buffer (pH-4.2) (37:63% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 260 nm. The retention time of the Azelnidipine and Telmisartan was found to be was 2.133, 3.692±0.02 min respectively. The method was validated according to ICH guidelines for linearity, sensitivity, accuracy, precision, specificity and robustness. The method produce linear responses in the concentration range of 20-60mg/ml of Azelnidipine and 10-30mg/ml of Telmisartan. The inter-day and intra-day precisions were found to be within limits. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
subject: Articles
  topic: section
subject: Azelnidipine
  topic: keywords
subject: Telmisartan
  topic: keywords
subject: RP-HPLC
  topic: keywords
subject: Validation
  topic: keywords
subject: Accuracy
  topic: keywords
subject: Precision
  topic: keywords
creator: Amulya E
creator: Suresh Ch
creator: Ayesha Jabeen
creator: Sahitya B
creator: Nikhitha M
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-07-10
publication date: 2025-07-10
starting page: 41
ending page: 45
doi: 10.30904/j.jpbmal.2025.4831

Integrated Analytical Quality by Design Approach for the Development and Validation of Liquid Chromatography Method for Simulataneous Estimation of Sulfadoxane and Pyrimethamine
type: item
description: This research evaluates a validated chromatographic method for analyzing sulfadoxine and pyrimethamine. A quadratic model was most effective for retention time analysis (p < 0.0001). System suitability met regulatory standards for resolution, theoretical plates, and tailing. The method was validated for assay, linearity, precision, intermediate precision, accuracy, LOD, and LOQ. Assay values were 99.79% (sulfadoxine) and 99.52% (pyrimethamine). Linearity showed strong correlation (r = 0.9996 and 0.9993, respectively). Precision was excellent (%RSD<1), and accuracy fell within 98–102%. LODs were 0.5µg/mL and 0.2µg/mL, while LOQs were 1.9 µg/mL and 0.8µg/mL. The method proved robust under varied conditions and is suitable for concurrent formulation analysis.
subject: Articles
  topic: section
subject: Chromatographic resolution
  topic: keywords
subject: Sulfadoxine
  topic: keywords
subject: Pyrimethamine
  topic: keywords
subject: Validated method
  topic: keywords
subject: Quadratic model
  topic: keywords
subject: Retention time analysis
  topic: keywords
subject: System suitability parameters
  topic: keywords
subject: Assay and linearity
  topic: keywords
creator: Vijaya Kumar Gampa
creator: Basavaling M Yalagatti
creator: Sravanthi B
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-06-12
publication date: 2025-06-12
starting page: 31
ending page: 36
doi: 10.30904/j.jpbmal.2025.4821

RP-HPLC Method Development and Validation for the simultaneous estimation of Meropenem and Vaborbactam in its pure and Dosage form
type: item
description: A robust and optimized chromatographic method for the simultaneous quantification of Meropenem and Vaborbactam was developed using Spurcil C18 Column (250×4.6mm, 5µm) with a flow rate of 1.0 ml/min, ensuring excellent peak separation and resolution. System suitability tests confirmed compliance with essential parameters such as resolution, tailing factor, and plate count, reinforcing the method's reliability. The retention times (RT) for Meropenem and Vaborbactam were 3.090 min and 4.215 min, respectively. Linearity assessments demonstrated strong correlation coefficients (>0.999), validating the accuracy of quantification across a concentration range of 10-50 µg/ml. Precision studies exhibited low %RSD values, confirming reproducibility, while accuracy investigations yielded recovery rates within 98-102%, ensuring method robustness. Further validation was performed to assess ruggedness and robustness, confirming method stability under varying chromatographic conditions, including flow rate and mobile phase composition variations. Despite these modifications, retention times remained consistent, reaffirming reproducibility. The method demonstrated high sensitivity, with limits of detection (LOD) and limits of quantification (LOQ) established at 0.01µg/ml for Meropenem and 0.02µg/ml for Vaborbactam. Intermediate precision studies verified assay reliability across different analytical systems and days, further validating its consistency. In conclusion, the developed HPLC method for Meropenem and Vaborbactam is precise, accurate, linear, sensitive, and robust, making it highly suitable for routine pharmaceutical analysis and quality control applications. Compliance with regulatory standards ensures reliable identification and quantification, providing an efficient and reproducible analytical solution for the quality assurance and validation of Meropenem and Vaborbactam formulations.
subject: Articles
  topic: section
subject: Meropenem
  topic: keywords
subject: Vaborbactam
  topic: keywords
subject: Chromatographic Method
  topic: keywords
subject: HPLC
  topic: keywords
subject: Retention Time
  topic: keywords
subject: System Suitability
  topic: keywords
subject: Linearity
  topic: keywords
subject: Accuracy
  topic: keywords
subject: Precision
  topic: keywords
subject: Robustness
  topic: keywords
subject: Pharmaceutical Analysis
  topic: keywords
subject: Quality Control
  topic: keywords
creator: Tahseen Sultana
creator: Saritha V
creator: Sujatha Dr.S
creator: Navyasri G
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-06-02
publication date: 2025-06-02
starting page: 26
ending page: 30
doi: 10.30904/j.jpbmal.2025.4813

RP-HPLC Method Development and Validation of a Stability Indicating Cidofovir in Its Pure and Dosage Form
type: item
description: High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of Cidofovir was done by waters HPLC with auto sampler and PDA detector. The buffer 1ml TFA in 1000ml water. Adjust this solution to pH 3 by using acid / base based on the PH of the resulted solution and Mix a mixture of above ACN 650ml (65%), 350 ml TFA (35%) and degas in ultrasonic water bath for 5 minutes. Filter through 0.45 µ filter under vacuum filtration. Inspire (150X4.6mm 5µm) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 230nm. The solutions were chromatographed at a constant flow rate of 1ml/min and Injection volume 20l, the run time 10min, the linearity range was found to lie from 20µg/ml to 50µg/ml of Cidofovir. The results obtained on the validation parameters met ICH and USP requirements. It inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis.
subject: Articles
  topic: section
subject: TFA
  topic: keywords
subject: ICH
  topic: keywords
subject: USP
  topic: keywords
subject: Cidofovir
  topic: keywords
subject: CAN
  topic: keywords
subject: HPLC
  topic: keywords
subject: PDA
  topic: keywords
creator: Malathi Batchu
creator: Priyanka K
creator: Pradeep Kumar M
creator: Srikanth Anumalagundam
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-05-10
publication date: 2025-05-10
starting page: 18
ending page: 25
doi: 10.30904/j.jpbmal.2025.4806

Screening the efficacy of compounds from Ghee to control cancer through GC-MS Analysis
type: item
description: Ghee, a clarified form of butter, holds a revered status in Ayurveda as the healthiest source of edible fat, known for its diverse therapeutic and nutritional benefits. Traditionally integrated into the daily Indian diet and religious rituals, ghee is considered essential for promoting longevity, enhancing memory, and supporting the nervous system. Its unique ability to act as a vehicle for transporting herbal constituents to deeper tissues further underscores its medicinal value. Ayurvedic texts suggest that ghee improves digestion, absorption, and assimilation, while also lubricating the nervous tissue and enhancing bodily flexibility. However, from a modern scientific perspective, caution is warranted regarding excessive consumption. Ghee is rich in saturated fatty acids (65%), monounsaturated fatty acids (32%), and polyunsaturated fatty acids (3%), which, in high intake, may elevate cholesterol levels and increase the risk of cardiovascular diseases. Additionally, the role of lipid peroxidation and free radical damage in chronic diseases and aging is well-documented, linking excessive fat intake to pathological conditions such as ischemia, inflammation, stroke, and carcinogenesis. Thus, while ghee offers numerous health benefits as per Ayurvedic tradition, its consumption should be balanced and moderated in the context of modern health guidelines.
subject: Articles
  topic: section
subject: Ghee
  topic: keywords
subject: Ayurveda
  topic: keywords
subject: Edible fat
  topic: keywords
subject: Longevity
  topic: keywords
subject: Nervous system
  topic: keywords
subject: Digestion
  topic: keywords
subject: Lipid peroxidation
  topic: keywords
subject: Free radicals
  topic: keywords
subject: Cardiovascular disease
  topic: keywords
subject: Saturated fatty acids
  topic: keywords
subject: Cholesterol
  topic: keywords
subject: Traditional medicine
  topic: keywords
creator: Siva Prasad K
creator: Manikanteswari B
creator: Mahesh G
creator: Gangadevi K
creator: Veeraraghavulu T
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-05-08
publication date: 2025-05-08
starting page: 14
ending page: 17
doi: 10.30904/j.jpbmal.2025.4804

Development of a Robust RP-HPLC Method for the Analysis of Ofloxacin and Nitazoxanide in Fixed-Dose Combination Products
type: item
description: A new method was established for simultaneous estimation of Ofloxacin and Nitazoxanide by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Ofloxacin and Nitazoxanide by using Xterra C18 5µm (4.6*250mm) column, flow rate was 1ml/min, mobile phase ratio was Phosphate buffer (0.05M) pH 4.6: ACN (55:45%v/v) (pH was adjusted with orthophosphoric acid), detection wavelength was 255nm. The instrument used was Shimadzu, model No. SPD-20MA LC+20AD, Software- LC-20 Solution. The retention times were found to be 2.399mins and 3.907mins. The % purity of Ofloxacin and Nitazoxanide was found to be 100.7% and 101.4% respectively. The system suitability parameters for Ofloxacin and Nitazoxanide such as theoretical plates and tailing factors were found to be 1.3, 5117.5and 1.4, 3877.3 the resolution was found to be 5.31. In compliance with ICH criteria, the analytical procedure was verified (ICH, Q2 (R1)) 4. According to the linearity investigation, the concentration ranges of ofloxacin and nitazoxanide were 20μg–100μg and 10μg–50μg. The correlation coefficient (r2) was 0.999 and 0.999, and the percentage mean recovery was 100% and 100.5%. The repeatability RSD was between 0.2 and 0.4 percent. For intermediate precision, the corresponding RSDs were 0.5 and 0.1
subject: Articles
  topic: section
subject: Ofloxacin
  topic: keywords
subject: Nitazoxanide
  topic: keywords
subject: RP-HPLC method
  topic: keywords
creator: Chiranjeevi J
creator: Mahesh M
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0
date: 2025-05-05
publication date: 2025-05-05
starting page: 07
ending page: 13
doi: 10.30904/j.jpbmal.2025.4795

Development and Validation of a New RP-HPLC Method for the Estimation of Citalopram in Tablet Dosage Forms
type: item
description: A novel, precise, and accurate reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of citalopram in tablet dosage forms. The method utilized an Agilent Eclipse XDB C18 column (150 x 4.6 mm, 5 µm) with a mobile phase consisting of acetate buffer (pH 4.5) and acetonitrile in a 65:35 v/v ratio, pumped at a flow rate of 1.0 mL/min. Detection was carried out at 240 nm, and the retention time for citalopram was found to be 3.727 minutes. The method demonstrated excellent linearity in the concentration range of 25, 150 µg/mL (r² = 0.999). Validation studies confirmed the method's precision, accuracy, specificity, and robustness, with recovery values ranging from 98.65% to 101.72%. The limits of detection (LOD) and quantification (LOQ) were determined to be 1.125 µg/mL and 3.375 µg/mL, respectively. The method was successfully applied to quantify citalopram in commercial tablet formulations, with results showing 100.1% recovery of the labeled claim. Forced degradation studies under acidic, basic, oxidative, thermal, photolytic, and neutral conditions revealed that the method could effectively separate citalopram from its degradation products, indicating its stability-indicating capability. The proposed method offers advantages over existing methods, including shorter analysis time (8 minutes), higher sensitivity, and a wider linearity range. It is concluded that this RP-HPLC method is suitable for routine quality control analysis of citalopram in pharmaceutical formulations.
subject: Articles
  topic: section
subject: Citalopram
  topic: keywords
subject: RP-HPLC
  topic: keywords
subject: Method Development
  topic: keywords
subject: Method Validation
  topic: keywords
subject: Tablet Dosage Form
  topic: keywords
subject: Forced Degradation Studies
  topic: keywords
creator: Dhilip Kumar K.S
creator: Mahesh M
rights: Copyright (c) 2025 Author https://creativecommons.org/licenses/by-nc/4.0/
date: 2025-05-02
publication date: 2025-05-02
starting page: 01
ending page: 06
doi: 10.30904/j.jpbmal.2025.4789