Synthesis, Characterization and Anthelmentic Activity of Pyridazine Derivative

Abstract

A series of novel pyridazinone and pyridazine chloride derivatives were synthesized and characterized using spectroscopic techniques including infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. All synthesized compounds displayed characteristic peaks consistent with their proposed structures. The compounds were subsequently evaluated for anthelmintic activity using standardized in vitro methods. The results demonstrated that all title compounds exhibited significant anthelmintic activity, with compounds C2 and C6, bearing electron-rich chloro substituents at the 4th position of the pyridazinone and pyridazine chloride nuclei, showing relatively marked anthelmintic potency compared to other analogues. Structure-activity relationship analysis indicated that electron-donating groups, particularly chloro groups, enhanced the anthelmintic efficacy of these compounds. These findings underscore the potential for further structural optimization through the introduction of diverse electron-withdrawing and electron-donating substituents at the 4th position of the pyridazinone and pyridazine chloride scaffolds. The present work suggests that rational molecular design incorporating such substituents may yield significantly more potent anthelmintic and antioxidant agents with improved therapeutic profiles. Future investigations should focus on synthesizing additional analogues with varied electronic properties to identify lead compounds for preclinical and clinical development.