Development and Validation of a New RP-HPLC Method for the Simultaneous Estimation of Moexipril and Hydrochlorothiazide acid in Pharmaceutical Dosage form as Per ICH guidlines

Authors

  • Narendar Reddy Department of Pharmaceutical Analysis, KGR Institute of Technology and Management, Rampally, Keesara(m), Medchal-Malkajgiri District, Hyderabad -501302, Telangana, India. Author
  • T. Rajesh Department of Pharmaceutical Analysis, KGR Institute of Technology and Management, Rampally, Keesara(m), Medchal-Malkajgiri District, Hyderabad -501302, Telangana, India. Author
  • Vijay Kumar Gampa Principal and Professor, KGR Institute of Technology and Management, Rampally, Keesara(m), Medchal-Malkajgiri District, Hyderabad -501302, Telangana, India. Author
  • B. Sravanthi Assistant Professor, Department of Pharmaceutical Analysis, Rampally, Keesara(m), Medchal-Malkajgiri District, Hyderabad -501302, Telangana, India. Author

DOI:

https://doi.org/10.30904/

Keywords:

Moexipril, Hydrochlorothiazide, Hypersil C18 column, validation, reliability, RP-HPLC, ICH guidelines

Abstract

This research work verified a HPLC technique for the concurrent identification of Moexipril & Hydrochlorothiazide in formulations. The technique demonstrated high specificity, with a resolution greater than 2 & % assay results of 101% for Moexipril &98.9% for Hydrochlorothiazide. Linearity was confirmed over the range of 20 to 100 µg per ml, with R² of 0.9999 & 0.9989, respectively. Precision was verified with %RSD values of 0.2% & 1.4%, & accuracy was supported by recovery studies yielding mean recoveries of 98.33% & 99.4%. Degradation studies indicated stability under various stress conditions, with minimal degradation (≤ 2.72%). Overall, the technique is robust & suitable for regular analysis in QC laboratories.

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Published

2024-12-17

Issue

Vol. 12 No. 01 (2024): IJCPS

Section

Articles

License

Copyright (c) 2024 Author

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Reddy , N., T, R., Gampa, V. K., & B, S. (2024). Development and Validation of a New RP-HPLC Method for the Simultaneous Estimation of Moexipril and Hydrochlorothiazide acid in Pharmaceutical Dosage form as Per ICH guidlines. International Journal of Chemistry and Pharmaceutical Sciences, 12(01), 34-42. https://doi.org/10.30904/