Design, Synthesis, Characterization, and Analysis of Antimicrobial Property of Novel Benzophenone Fused Azetidinone Derivatives through In-Silico Approach

Abstract

A sequence of novel 2-(4-benzoyl-2-methyl-phenoxy)-N-(3-chloro-2-oxo-4-phenyl-azetidin-1-yl)-acetamide analogues 9(a–n) were synthesized through a multistep process. The newly developed compounds were thoroughly characterized, and their antimicrobial activities were evaluated using disc diffusion and broth dilution methods. Additionally, all compounds in the series (9a–n) were tested against various bacterial and fungal strains, with Ketoconazole, Chloramphenicol, and Amoxicillin serving as reference drugs. Among them, compounds 9a, 9e, and 9g exhibited significant inhibition against the tested strains. Furthermore, molecular docking studies were conducted on the most potent compounds to analyze their three-dimensional geometric interactions with target proteins.